Concentration of Hydrogen Peroxide The different concentrations of hydrogen peroxide that I made could not have been exactly accurate because this would have meant that the volume of gas evolved would have increased in equal steps, which it did not. Pour enough hydrogen peroxide into the cup so that potato is submerged and observe.
Keep one section raw and at room temperature. Discs of potato react too slowly. For example, the final average volumes of gas were as follows: Then the rate of reaction is calculated. This should tell me whether the reaction is indeed a first-order reaction, or if a curve of best fit is required.
Surface Area of Yeast Molecules I ground up the yeast to try to make the surface area Catalase hydrogen peroxide experiment coursework similar as possible because surface area is a major factor in my experiment.
Teaching notes Note the units for measuring the concentration of hydrogen peroxide — these are not SI units.
This would have affected the number of molecules of hydrogen peroxide present, which in turn would have affected the number of collisions between enzyme and substrate molecules.
This is shown when oxygen stops being produced and the same results are recorded five times. Go further in this experiment by looking at a very small sample of potato combined with hydrogen peroxide under a microscope!
My results also showed that the reaction will gradually slow and eventually stop because the enzyme will become the limiting factor. If my replicates had not been so close I would have had to change the tube.
Be aware of pressure building up if reaction vessels become blocked. However, I do not believe the substrate concentrations were significantly different because my repeats were mostly concordant, so a similar amount of oxygen was produced which must mean that there was a similar number of substrate molecules in each concentration.
Set out in brown bottles Note 1. I tried to make this as accurate as possible by keeping my eyes level with the gas syringe. Investigating an enzyme-controlled reaction: This means that the reaction a first-order reaction, so the rate is proportional to the concentration.
The accuracy of the results showed that this factor did not distort the results too much, and so a similar amount of substrate molecules were present in each reaction. For example, if there were more molecules of yeast, the rate of reaction would increase because there would be more collisions between enzyme and substrate molecules.
The bubbling reaction you see is the metabolic process of decomposition, described earlier. The reaction is exothermic. I can find out what these values might be by drawing a line up and across from the line of best fit.
Conclusions Catalase acts as the catalyzing enzyme in the decomposition of hydrogen peroxide.
In addition, your access to Education. I believe that my results were also relatively reliable because as the concentration decreased the volume of oxygen produced also decreased.
Values below 22 cm3 show that oxygen has escaped, or the hydrogen peroxide has not fully reacted, or the hydrogen peroxide concentration is not as expected.
The line of best fit also suggests values of concentrations which I have not investigated. I believe that the data also shows strong positive correlation, and there are few outliers, which shows that my results are accurate.
To prevent this I had to dry out the barrel and syringe before commencing the procedure. This means that having the same surface area of yeast in each reaction is very important in ensuring a fair test because the number of molecules exposed to collisions must be the same.
Another factor which was hard to measure was the volume of gas produced, because some of the higher concentration reactions were very fast, so it was hard to read the correct values every time.
Ask students to explain how values over 22 cm3 could happen. I did this by only using pipettes, which have a very small apparatus error when compared to beakers.
Do not return solution to stock bottles, because contaminants may cause decomposition and the stock bottle may explode after a time.CATALASE AND HYDROGEN PEROXIDE Introduction My coursework is on catalase activity. This is done using hydrogen peroxide and two independent variables of my choice.
I carried out a preliminary experiment so that I got a basic idea of what I was doing and what to expect. Let us write or edit the lab report on your topic "Action Of Enzyme Catalase On Hydrogen Peroxide" with a personal 20% discount.
Hydrogen peroxide (H 2 O 2) is a by-product of respiration and is made in all living cells. Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell.
Cells make the enzyme catalase to remove hydrogen peroxide. enzyme Catalase. In this experiment I will be using yeast as a source [tags: GCSE Chemistry Coursework Investigation] - The Effect Of Catalase On Hydrogen Peroxide Planning Catalase is an enzyme found in the blood and in most living cells its job is to speed up the decomposition of hydrogen peroxide into water and oxygen.
2H2O2. Apr 30, · This is because as there are progressively fewer molecules of hydrogen peroxide there will be fewer collisions between the substrate and enzyme molecules (catalase in yeast), leading to a decrease in enzyme-substrate complexes killarney10mile.coms: 5.
The test was carried out, using potato as a source of catalase and reacting it with hydrogen peroxide and ph. Five different ranges were used for the two variables and each test was repeated 3 times with new pieces of potato discs and the volume of oxygen was measured.Download